Bioluminescent assay for quantitation microRNAs.
نویسندگان
چکیده
منابع مشابه
Macropinosome quantitation assay
In contrast to phagocytosis, macropinocytosis is not directly initiated by interactions between cell surface receptors and cargo ligands, but is a result of constitutive membrane ruffling driven by dynamic remodelling of cortical actin cytoskeleton in response to stimulation of growth factor receptors. Wang et al. (2010) [13] developed a reliable assay that allows quantitative assessment of the...
متن کاملImmunosorbent Assay for Quantitation of Antibody
Antibodies to the Ro/SSA antigen occur in patients with systemic lupus erythematosus and Sjogren's syndrome. An immunoaffinity method for the preparation of electrophoretically homogeneous Ro/SSA antigen is described. Several molecular properties of the antigen have been determined. The native RNA protein particle has a molecular weight of 100,000 D determined by gel filtration. Sodium dodecyl ...
متن کاملA simple, fully enzymic bioluminescent assay for triglycerides in serum.
In this fully enzymic bioluminescent assay, triglycerides are cleaved by lipase (EC 3.1.1.3) and carboxylesterase (EC 3.1.1.1), and the glycerol obtained is phosphorylated with ATP and glycerol kinase (EC 2.7.1.30). The ATP consumed in the latter reaction is determined by the firefly luciferin-luciferase reaction, and corresponds to the concentration of triglycerides. All the enzymic reactions ...
متن کاملA bioluminescent assay for the sensitive detection of proteases.
A bioluminescent general protease assay was developed using a combination of five luminogenic peptide substrates. The peptide-conjugated luciferin substrates were combined with luciferase to form a homogeneous, coupled-enzyme assay. This single-reagent format minimized backgrounds, gave stable signals, and reached peak sensitivity within 30 min. The bioluminescent assay was used to detect multi...
متن کاملThe MAO-Glo Assay: A bioluminescent-coupled assay for monoamine oxidase activity
Although cytochrome P450 enzymes are responsible for the majority of drug metabolism reactions, the monoamine oxidases (MAOs) are arguably the second-most important phase I biotransformation enzymes. MAOs catalyze the oxidative deamination of a number of biogenic and xenobiotic amines to the corresponding aldehydes with consumption of O2 and production of H2O2 (1). These flavoenzymes are locate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2009
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.23.1_supplement.846.2